Characterization of monoclonal antibodies that specifically recognize the palm subdomain of hepatitis C virus nonstructural protein 5B polymerase.

Title:

Characterization of monoclonal antibodies that specifically recognize the palm subdomain of hepatitis C virus nonstructural protein 5B polymerase.

Dataset

identifier:	1313611
description:	epitope description:DSTVTENDIRVEESIYQCCDLAPEARQAIKSLTERLY antigen name:Genome polyprotein host organism:Mus musculus antibody name:16A₉C₉
aggregation:	instance of dataset
availability:	available
primaryPublications:	11325472

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1001946
landingPage:	http://www.iedb.org/assay/1313611

Publication

type:	Literature
publicationVenue:	Virus Res
dates:	2001

Dimension

study type:	b cell assays
subject species:	Hepatitis C virus (isolate H77)

Person

fullName:	P Ingravallo F Lahser E Xia B Sodowich V C Lai Z Hong W Zhong

Data Type

method:	western blot

Study

name:	Characterization of monoclonal antibodies that specifically recognize the palm subdomain of hepatitis C virus nonstructural protein 5B polymerase.
description:	HCV/GBV-B chimeric proteins were used to map the epitope in HCV NS5B for the epitope-specific monoclonal antibody. This approach was based on the fact that GBV-B NS5B possesses a high degree of homology to HCV NS5B but lacks reactivity with the monoclonal antibody. The monoclonal antibody reacted with HCV NS5B 231-372, but did not react with 267-372. The results suggested that the epitope is located between amino acid 231 and 267 of HCV NS5B.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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