Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1001974 |
| landingPage: |
http://www.iedb.org/assay/1314366 |
| type: |
Literature
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| publicationVenue: |
J Gen Virol
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| dates: |
1994
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| study type: | b cell assays |
| subject species: | Hepatitis E virus (strain Burma) |
| fullName: |
Khudyakov YuE
N S Khudyakova
D L Jue
T W Wells
N Padhya
H A Fields
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| method: |
ELISA
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| name: |
Comparative characterization of antigenic epitopes in the immunodominant region of the protein encoded by open reading frame 3 in Burmese and Mexican strains of hepatitis E virus.
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| description: |
The sequence is from the C-terminal region of the HEV ORF 3 protein.
Human anti-HEV sera from an outbreak in Turkmenistan in 1985 were assayed against a panel of overlapping hexamer sequences covering residues 91-123 of the ORF 3 protein of HEV strain Burma. A Lys residue was added to the N terminus and 2 Gly residues were added to the C terminus of the hexamers to improve solubility and plate adsorption. Almost 40% of anti-HEV(T) sera (n = 16) reacted with the epitope. B22 was recognized by 8 of 37 anti-HEV sera from outbreaks in Mexico, Turkmenistan, and Kenya and contains the most immunoreactive strain-specific epitope in this region.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
