Identification of D motif epitopes in Staphylococcus aureus fibronectin-binding protein for the production of antibody inhibitors of fibronectin binding.

Title:

Identification of D motif epitopes in Staphylococcus aureus fibronectin-binding protein for the production of antibody inhibitors of fibronectin binding.

Dataset

identifier:	1316317
description:	epitope description:EEDTEEDKPK antigen name:Fibronectin-binding protein A host organism:Oryctolagus cuniculus New Zealand White antibody name:anti-D1-3
aggregation:	instance of dataset
availability:	available
primaryPublications:	9009310

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1002025
landingPage:	http://www.iedb.org/assay/1316317

Publication

type:	Literature
publicationVenue:	Infect Immun
dates:	1997

Dimension

study type:	b cell assays
subject species:	Staphylococcus aureus

Person

fullName:	Q Sun G M Smith C Zahradka M J McGavin

Data Type

method:	ELISA

Study

name:	Identification of D motif epitopes in Staphylococcus aureus fibronectin-binding protein for the production of antibody inhibitors of fibronectin binding.
description:	The peptide corresponds to amino acids 9-18 of the D1 motif. Epitope mapping of affinity-purified anti-D1-3 was performed using 24 overlapping decapeptides spanning the D1 motif and the N-terminal 18 residues of D2. Each peptide contained an N-terminal biotin and a 4 residue spacer SGSG. Anti-D1-3 reacted strongly with the epitope, which is included in a cluster of acidic amino acids spanning residues 7-20 of the D1 motif.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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