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Title: Evaluation of the latency-associated nuclear antigen (ORF73) of Kaposi's sarcoma-associated herpesvirus by peptide mapping and bacterially expressed recombinant western blot assay.
identifier: 1316688
description:
epitope description:DEQEQQEEQEQQEEQEQ
antigen name:Protein ORF73
host organism:Homo sapiens
aggregation:
instance of dataset
availability:
available
primaryPublications: 10882613
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1001996
landingPage: http://www.iedb.org/assay/1316688
type:
Literature
publicationVenue:
J Infect Dis
dates:
2000
study type: b cell assays
subject species: Human gammaherpesvirus 8
fullName:
S J Olsen
R Sarid
Y Chang
P S Moore
method:
ELISA
name:
Evaluation of the latency-associated nuclear antigen (ORF73) of Kaposi's sarcoma-associated herpesvirus by peptide mapping and bacterially expressed recombinant western blot assay.
description:
A positive result was scored if the ratio of optical density of patient sera to the average of two KSHV-seronegative blood donors for the peptide greater than 2. Lysates of E. coli transformed with a plasmid encoding full length ORF73 (LANA) or purified protein can be recognized by patient sera by Western Blot (67% positive).

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