Clearance and prevention of prion infection in cell culture by anti-PrP antibodies. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Clearance and prevention of prion infection in cell culture by anti-PrP antibodies.

Dataset

identifier:	1327119
description:	epitope description:GTHNQWNKPSKPKTNLK antigen name:Major prion protein host organism:Mus musculus PrP null antibody name:6D11
aggregation:	instance of dataset
availability:	available
primaryPublications:	16817866

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1002125
landingPage:	http://www.iedb.org/assay/1327119

Publication

type:	Literature
publicationVenue:	Eur J Neurosci
dates:	2006

Dimension

study type:	b cell assays
subject species:	Mus musculus

Person

fullName:	Joanna Pankiewicz Frances Prelli Man-Sun Sy Richard J Kascsak Regina B Kascsak Daryl S Spinner Richard I Carp Harry C Meeker Marcin Sadowski Thomas Wisniewski

Data Type

method:	biological activity

Study

name:	Clearance and prevention of prion infection in cell culture by anti-PrP antibodies.
description:	N2a cells were infected with the mouse-adapted scrapie strain 22L. Culturing N2a/22L cells in the presence of epitope-specific MAb 6D11 abrogated PrP^sc without affecting the level of PrP^c, as detected by immunoblotting PK-resistant PrP^sc and total untreated PrP in cell lysates.The effect of treatment with Mab 6 D11 persisted for 14 days in the absence of antibody. MAb 6D11 did not have a trophic or toxic effect on N2a/22L cells. Preincubation of N2a cells prior to inoculation or preincubation of the inoculum (brain homogenate from 22L-infected mice) with MAb 6D11 also resulted in reduced PrP^sc levels.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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