Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1002012 |
| landingPage: |
http://www.iedb.org/assay/1329826 |
| type: |
Literature
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| publicationVenue: |
Eur J Biochem
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| dates: |
2001
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| study type: | b cell assays |
| subject species: |
| fullName: |
O Minenkova
N Gargano
A De Tomassi
F Bellintani
A Pucci
P Fortugno
E Fuscaldi
A Pessi
M Rapicetta
M Miceli
P Iudicone
R Cortese
F Felici
P Monaci
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| method: |
ELISA
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| name: |
ADAM-HCV, a new-concept diagnostic assay for antibodies to hepatitis C virus in serum.
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| description: |
The peptide is derived from a phage-displayed library of mutagenized HCV sequences. The N-terminal (AEGEF) and C-terminal (DPAK) flanking sequences are derived from the phage pVIII major capsid protein.
The epitope was identified from a phage-displayed peptide library of mutagenized HCV sequences by multiple rounds of screening using HCV-positive human sera. Of 21 HCV-positive sera, 100% recognized the epitope with reactivity of 3 SD or greater than the mean negative control value. The epitope was recognized by 9 of 31 sera diagnosed as indeterminate by a commercial HCV assay. The authors concluded that these 9 sera are HCV-positive because of reactivity with 2 or more peptides recognized by HCV-positive sera.The carrier consisted of an octabranching multiple antigen peptide.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
