ADAM-HCV, a new-concept diagnostic assay for antibodies to hepatitis C virus in serum. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

ADAM-HCV, a new-concept diagnostic assay for antibodies to hepatitis C virus in serum.

Dataset

identifier:	1329859
description:	epitope description:AEGEFATAPPSHYSWDPAK host organism:Homo sapiens antibody name:HCV-positive sera
aggregation:	instance of dataset
availability:	available
primaryPublications:	11532012

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1002012
landingPage:	http://www.iedb.org/assay/1329859

Publication

type:	Literature
publicationVenue:	Eur J Biochem
dates:	2001

Dimension

study type:	b cell assays
subject species:

Person

fullName:	O Minenkova N Gargano A De Tomassi F Bellintani A Pucci P Fortugno E Fuscaldi A Pessi M Rapicetta M Miceli P Iudicone R Cortese F Felici P Monaci

Data Type

method:	ELISA

Study

name:	ADAM-HCV, a new-concept diagnostic assay for antibodies to hepatitis C virus in serum.
description:	The peptide is derived from a phage-displayed library of mutagenized HCV sequences. The N-terminal (AEGEF) and C-terminal (DPAK) flanking sequences are derived from the phage pVIII major capsid protein. The epitope was identified from a phage-displayed peptide library of mutagenized HCV sequences by multiple rounds of screening using HCV-positive human sera. Of 33 HCV-positive sera, 27% recognized the epitope with reactivity of 3 SD or greater than the mean negative control value. The epitope was not recognized by any of 31 sera diagnosed as indeterminate by a commercial HCV assay.The carrier consisted of an octabranching multiple antigen peptide.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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