Effect of sequence variation in Plasmodium falciparum histidine- rich protein 2 on binding of specific monoclonal antibodies: Implications for rapid diagnostic tests for malaria.

Title:

Effect of sequence variation in Plasmodium falciparum histidine- rich protein 2 on binding of specific monoclonal antibodies: Implications for rapid diagnostic tests for malaria.

Dataset

identifier:	1333776
description:	epitope description:YAHHAHHA antigen name:Histidine-rich protein III host organism:Mus musculus antibody name:1E1-A9
aggregation:	instance of dataset
availability:	available
primaryPublications:	16891491

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1002289
landingPage:	http://www.iedb.org/assay/1333776

Publication

type:	Literature
publicationVenue:	J Clin Microbiol
dates:	2006

Dimension

study type:	b cell assays
subject species:	Plasmodium falciparum

Person

fullName:	Nelson Lee Joanne Baker Kathy T Andrews Michelle L Gatton David Bell Qin Cheng James McCarthy

Data Type

method:	ELISA

Study

name:	Effect of sequence variation in Plasmodium falciparum histidine- rich protein 2 on binding of specific monoclonal antibodies: Implications for rapid diagnostic tests for malaria.
description:	This epitope occurs in other positions (179-186, 200-207) within the source protein. The epitope of the monoclonal antibody 1E1-A9 was determined by epitope mapping using synthetic peptides derived from the source protein (histidine rich protein, from 46 isolates of Plasmodium falciparum); substitutions of D or V for the first or last amino acid, respectively, still allows binding to mAb 1E1-A9. The minimal epitope, determined with glycine scanning, is AHHAHHV.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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