Identification and chemical synthesis of a tandemly repeated immunogenic region of Plasmodium knowlesi circumsporozoite protein.

Title:

Identification and chemical synthesis of a tandemly repeated immunogenic region of Plasmodium knowlesi circumsporozoite protein.

identifier:	1334042
description:	epitope description:QAQGDGANAGQP antigen name:Circumsporozoite (CS) protein host organism:Mus musculus antibody name:2G3
aggregation:	instance of dataset
availability:	available
primaryPublications:	6193427

study type:	b cell assays
subject species:	Plasmodium knowlesi strain H

fullName:	G N Godson J Ellis P Svec D H Schlesinger V Nussenzweig

method:	radio immuno assay (RIA)

name:	Identification and chemical synthesis of a tandemly repeated immunogenic region of Plasmodium knowlesi circumsporozoite protein.
description:	The authors deduce that mAb 2G3 binds to the epitope because a 24-mer peptide containing two tandem repeats of the epitope binds two molecules of antibody in a two-site immunoradiometric assay. The 12-mer epitope cannot bind two molecules of 2G3 simultaneously. The 24-mer peptide also bound to immobilzed mAbs 5H8, 8B8, and 8A8 made against P. knowlesi circumsporozoite protein. The authors conclude that tandemly repeated epitopes constitute the correct deduced sequence of a partial cDNA clone (pEG81) of the P. knowlesi circumsporozoite protein.

name:	iedb
homePage:	http://www.iedb.org

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