Rhoptry-associated protein 1-binding monoclonal antibody raised against a heterologous peptide sequence inhibits Plasmodium falciparum growth in vitro.

Title:

Rhoptry-associated protein 1-binding monoclonal antibody raised against a heterologous peptide sequence inhibits Plasmodium falciparum growth in vitro.

Dataset

identifier:	1338820
description:	epitope description:SFLENKSSVDDGNIN antigen name:Rhoptry-associated protein 1, RAP1 host organism:Mus musculus antibody name:SP5.2
aggregation:	instance of dataset
availability:	available
primaryPublications:	11254620

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1002638
landingPage:	http://www.iedb.org/assay/1338820

Publication

type:	Literature
publicationVenue:	Infect Immun
dates:	2001

Dimension

study type:	b cell assays
subject species:	Plasmodium falciparum

Person

fullName:	R Moreno F Pö ltl-Frank D Stü ber H Matile M Mutz N A Weiss G Pluschke

Data Type

method:	ELISA

Study

name:	Rhoptry-associated protein 1-binding monoclonal antibody raised against a heterologous peptide sequence inhibits Plasmodium falciparum growth in vitro.
description:	This is part of a set of overlapping 15-mer peptides covering the P. falciparum rhoptry-associated protein 1 (RAP-1) sequence from residues 23 to 294. Epitope mapping analysis was performed in order to identify reactive residues between p191-224 on RAP-1. Synthetic 15-mer peptides spanning the sequence between 23-294 were generated for this analysis. Epitope assignments by peptide mapping were consistent with results obtained by Western blotting with the truncated rRAP-1 molecules. The linear sequence YKYSL (218-222) was found to be the target of anti-35.1 mAbs.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.

Related Publications

Feedback?