Isolation of peptides that mimic epitopes on a malarial antigen from random peptide libraries displayed on phage.

Title:

Isolation of peptides that mimic epitopes on a malarial antigen from random peptide libraries displayed on phage.

identifier:	1337802
description:	epitope description:CFDYAPYVSAVDDIC host organism:Mus musculus antibody name:18/2
aggregation:	instance of dataset
availability:	available
primaryPublications:	10456916

study type:	b cell assays
subject species:

fullName:	C G Adda L R F Anders M Foley

method:	ELISA

name:	Isolation of peptides that mimic epitopes on a malarial antigen from random peptide libraries displayed on phage.
description:	This peptide mimics epitopes on Plasmodium falciparum ring-infected erythrocyte surface antigen (RESA). The motif (S/T)AVDD seems to be important for binding to the monoclonal antibody 18/2 since it also occurs in other mimotopes (that bind to 18/2). The monoclonal antibody, 18/2, raised against the C-terminal (residues 893-1073) of ring infected erythrocyte surface antigen (RESA) from Plasmodium falciparum, captured the mimotope from a random peptide phage display library. Subsequently, the mimotope was shown to bind to 18/2 by ELISA and western blot as linear or cyclized peptides, as well as after reduction and alkylation. The mimotope inhibited the binding of 18/2 to a recombinant form of RESA (aa 322-1073) as well as to infected erythrocytes, and is itself inhibited by the RESA repeats (EENV)₈, (EENVEHDA)₄ and (DDEHVEEPTVA)₂.

name:	iedb
homePage:	http://www.iedb.org

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.