Immunogenicity of a hybrid Plasmodium falciparum malaria antigen. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Immunogenicity of a hybrid Plasmodium falciparum malaria antigen.

Dataset

identifier:	1340046
description:	epitope description:NANPNANPNANPNANPNA antigen name:Circumsporozoite (CS) protein host organism:Oryctolagus cuniculus antibody name:anti-CSP repeat
aggregation:	instance of dataset
availability:	available
primaryPublications:	8341580

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1002754
landingPage:	http://www.iedb.org/assay/1340046

Publication

type:	Literature
publicationVenue:	Parasitology
dates:	1993

Dimension

study type:	b cell assays
subject species:	Plasmodium falciparum

Person

fullName:	M J Lockyer H Cooper J Tite W Rowan J S Crowe

Data Type

method:	ELISA

Study

name:	Immunogenicity of a hybrid Plasmodium falciparum malaria antigen.
description:	The circumsporozoite protein contains multiple tandem repeats of the sequence NANP. The epitope was recognized by sera from rabbits immunized with a construct containing the epitope within the sequence (NANP)₂₆ derived from the P. falciparum circumspororzoite protein and containing the C-terminal 293 amino acids of the major merozoite surface antigen (PMMSA). Immunization using Freund's adjuvant produced the highest titer antisera. The response to the epitope after the first immunization with baculovirus-expressed antigen was not increased after boosting, whereas a boosting response to a bacteria-expressed antigen was observed. For the ELISA capture antigen, the CSP peptide was synthesized with an N-terminal Cys residue and coupled to ovalbumin using glutaraldehyde.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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