Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1002850 |
| landingPage: |
http://www.iedb.org/assay/1340957 |
| type: |
Literature
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| publicationVenue: |
Vaccine
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| dates: |
1998
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| study type: | b cell assays |
| subject species: |
| fullName: |
E H Nardin
J M Calvo-Calle
G A Oliveira
P Clavijo
R Nussenzweig
R Simon
W Zeng
K Rose
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| method: |
ELISA
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| name: |
Plasmodium falciparum polyoximes: highly immunogenic synthetic vaccines constructed by chemoselective ligation of repeat B-cell epitopes and a universal T-cell epitope of CS protein.
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| description: |
The CSP B repeat epitopes were combined in tetrabranched, di- and tri-epitope constructs with and without a T-helper epitope. The combined peptide sequence was derived from the CS protein of P. falciparum (NF54).
In high responder C57BL/10 (H-2b) mice, the T1B4 polypeptide, constructed by either chemoligation (polyoxime) or stepwise synthesis (MAP) were immunogenic with or without adjuvant (Alum). In the absence of adjuvant, peak titers were 6-fold higher for polyoxime the T1B4 immunized mice. Inclusion of the Pam3Cys adjuvant in the T1B4 polyoxime construct significantly enhanced its immunogenicity. A single injection elicited positive responses in all three mouse strains tested. Similarly, very high titers were measured for Pam3Cys-T1B4 polyoxime immunized C57BL/10 mice by immunofluorescence assay (IFA).
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
