Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1002850 |
| landingPage: |
http://www.iedb.org/assay/1340968 |
| type: |
Literature
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| publicationVenue: |
Vaccine
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| dates: |
1998
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| study type: | b cell assays |
| subject species: |
| fullName: |
E H Nardin
J M Calvo-Calle
G A Oliveira
P Clavijo
R Nussenzweig
R Simon
W Zeng
K Rose
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| method: |
ELISA
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| name: |
Plasmodium falciparum polyoximes: highly immunogenic synthetic vaccines constructed by chemoselective ligation of repeat B-cell epitopes and a universal T-cell epitope of CS protein.
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| description: |
The CSP B repeat epitopes were combined in tetrabranched, di- and tri-epitope constructs with and without a T-helper epitope. The combined peptide sequence was derived from the CS protein of P. falciparum (NF54).
In intermediate responder A/J (H-2a) mice, only the T1B4 polypeptide, constructed by MAP and delivered in Alum were immunogenic. with or without adjuvant (Alum). Inclusion of the Pam3Cys adjuvant in the T1B4 polyoxime construct significantly enhanced its immunogenicity. A single injection elicited positive responses in all three mouse strains tested. Similarly, intermediate titers were measured for Pam3Cys-T1B4 polyoxime immunized A/J mice by immunofluorescence assay (IFA).
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
