Antibodies to malaria peptide mimics inhibit Plasmodium falciparum invasion of erythrocytes. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Antibodies to malaria peptide mimics inhibit Plasmodium falciparum invasion of erythrocytes.

Dataset

identifier:	1341658
description:	epitope description:LASLRKAFADTVPVRPPSNY host organism:Rattus norvegicus LOU/M antibody name:4G2dc1
aggregation:	instance of dataset
availability:	available
primaryPublications:	14742560

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1002888
landingPage:	http://www.iedb.org/assay/1341658

Publication

type:	Literature
publicationVenue:	Infect Immun
dates:	2004

Dimension

study type:	b cell assays
subject species:

Person

fullName:	Joanne L Casey Andrew M Coley Robin F Anders Vince J Murphy Karen S Humberstone Alan W Thomas Michael Foley

Data Type

method:	ELISA

Study

name:	Antibodies to malaria peptide mimics inhibit Plasmodium falciparum invasion of erythrocytes.
description:	This peptide is derived from a random 20-mer peptide library. It mimics a conformational epitope on P.falciparum AMA-1 protein, recognized by the inhibitory rat mAb 4G2dc1. The library was constructed with random 20-mer peptides fused to the N-terminus of protein III of phage M13. Isolated after 6 rounds of panning with mAb 4G2dc1 (directed towards an undefined conformational epitope on plasmodium falciparum AMA-1, with inhibitory activity on merozoite invasion into erythrocytes), the phage particle carrying this peptide is specifically recognized in ELISA by the selecting mAb. Binding could be inhibited by soluble AMA-1 protein, implying a true mimic of the 4Gsdc1 epitope by this peptide.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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