In human malaria protective antibodies are directed mainly against the Lys-Glu ion pair within the Lys-Glu-Lys motif of the synthetic vaccine SPf 66.

Title:

In human malaria protective antibodies are directed mainly against the Lys-Glu ion pair within the Lys-Glu-Lys motif of the synthetic vaccine SPf 66.

Dataset

identifier:	1349300
description:	epitope description:YSLFQKEK antigen name:Merozoite surface protein 1 host organism:Homo sapiens
aggregation:	instance of dataset
availability:	available
primaryPublications:	1557226

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1002783
landingPage:	http://www.iedb.org/assay/1349300

Publication

type:	Literature
publicationVenue:	Parasite Immunol
dates:	1992

Dimension

study type:	b cell assays
subject species:	Plasmodium falciparum

Person

fullName:	A Molano C Segura F Guzman D Lozada M E Patarroyo

Data Type

method:	antigen inhibition

Study

name:	In human malaria protective antibodies are directed mainly against the Lys-Glu ion pair within the Lys-Glu-Lys motif of the synthetic vaccine SPf 66.
description:	Sera from individuals immunized with the synthetic malaria vaccine, SPf66, which contains the epitope, recognized the epitope by ELISA. Deletion of the C-terminal amino acids MVL had little effect on recognition indicating that these residues are not crucial for antibody binding (thus, minimal epitope). Substitutions on the KEK motif within the epitope showed the following allowable changes that do not significantly affect recognition: 1) R for K48 but not G, A, I, V or L, 2) D for E49 but not L, A, K or V, and 3) K50 may be deleted. Antibody recognition was tested by competitive ELISA with the substituted peptides competing against the epitope.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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