Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1003131 |
| landingPage: |
http://www.iedb.org/assay/1348955 |
| type: |
Literature
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| publicationVenue: |
J Biol Chem
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| dates: |
2004
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| study type: | b cell assays |
| subject species: |
| fullName: |
Roshni Sundaram
Marcus P Lynch
Sharad V Rawale
Yiping Sun
Mirdad Kazanji
Pravin T P Kaumaya
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| method: |
biological activity
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| name: |
De novo design of peptide immunogens that mimic the coiled coil region of human T-cell leukemia virus type-1 glycoprotein 21 transmembrane subunit for induction of native protein reactive neutralizing antibodies.
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| description: |
This peptide is derived from the central region of the coiled coil segment of GP21 trasmembrane subunit of HTLV-1. This region is involved in the fusion of viral and cellular membranes and syncytia formation between infected cells. The peptide was modified with five L substitutions (at positions 4,8,15,18 and 25) to increase the hydrophobic interaction that stabilizes the coiled coil. When used as an isolated peptide, the C-terminus was also amidated.
Mouse antisera was raised to a construct where this peptide is linked in a 3meric form to facilitate the interaction into a native coiled-coil structure. The antisera is shown to recognize whole HTLV-1 lysates in ELISA, and to inhibit syncytia formation in vitro. Purified antibodies from the sera also shows inhibitory activity on syncytia formation.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
