Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1003103 |
| landingPage: |
http://www.iedb.org/assay/1347049 |
| type: |
Literature
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| publicationVenue: |
Parasitology
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| dates: |
1997
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| study type: | b cell assays |
| subject species: | Plasmodium falciparum |
| fullName: |
J Iqbal
N Siripoon
V G Snounou
P Perlmann
K Berzins
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| method: |
immuno staining
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| name: |
Plasmodium falciparum: selection of parasite subpopulations with decreased sensitivity for antibody-mediated growth inhibition in vitro.
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| description: |
The ring-infected erythrocyte surface antigen (RESA/Pf155) contains multiple tandem repeats of the sequence EENV.
The concentration of epitope-specific rabbit antibody R20 required to detect P. falciparum parasites was not affected by continual growth of parasites in culture for 12 weeks with R20 or a mAb to a different epitope or subsequent culture without the antibody. Parasites were detected by indirect immunofluorescence of unfixed and air-dried parasite-infected red blood cells or glutaraldehyde fixed air-dried monolayers of P. falciparum-infected erythrocytes. The authors conclude that antibody-selection presssure did not change antigen expression.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
