Identification and characterization of a C(K/R)TC motif as a common epitope present in all subtypes of hepatitis B surface antigen.

Title:

Identification and characterization of a C(K/R)TC motif as a common epitope present in all subtypes of hepatitis B surface antigen.

identifier:	1350108
description:	epitope description:CKTC antigen name:Large envelope protein host organism:Mus musculus BALB/c antibody name:H166
aggregation:	instance of dataset
availability:	available
primaryPublications:	8617960

study type:	b cell assays
subject species:	Hepatitis B virus

fullName:	X Qiu P Schroeder D Bridon

method:	antigen inhibition

name:	Identification and characterization of a C(K/R)TC motif as a common epitope present in all subtypes of hepatitis B surface antigen.
description:	The antigen used was a cyclic peptide formed by intramolecular disulfide bridging between C121 and C124. The cyclic peptide was more potent because it contains a loop conformation that is closer to the native structure of the a determinant on HBsAg than that of the linear peptide.In another experiment, a set of alanine-substituted analogues of the cyclic peptide were synthesized and tested by compeptitive ELISA. Substitutions of C121 and C124 with Ala completely abrogate the binding to mAb H166.

name:	iedb
homePage:	http://www.iedb.org

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