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identifier: 1351584
description:
epitope description:DRLLTTLSGPAQ
host organism:Bos taurus
antibody name:affinity-purified anti-p34
aggregation:
instance of dataset
availability:
available
primaryPublications: 14629623
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1003307
landingPage: http://www.iedb.org/assay/1351584
type:
Literature
publicationVenue:
Scand J Immunol
dates:
2003
study type: b cell assays
subject species:
fullName:
M Ostrowski
S L Mundo
N B Harris
R G Barletta
O J Lopez
method:
phage display
name:
B-cell epitopes in the immunodominant p34 antigen of mycobacterium avium ssp. paratuberculosis recognized by antibodies from infected cattle.
description:
The epitope shares a similar motif TTxxGP with residues 290-295 (SSPSGP) of the M. paratuberculosis p34 membrane protein.
The epitope was selected in a screen of a 12-mer phage display library using antibodies from M. paratuberculosis-infected cattle. The antibodies were affinity-purified with a recombinant C-terminal fragment (p-34cx) of the M. paratuberculosis p34 membrane protein, in which residues 290-295 (SSPSGP) share a similar motif with the epitope (TTxxGP).

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