Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1003185 |
| landingPage: |
http://www.iedb.org/assay/1353689 |
| type: |
Literature
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| publicationVenue: |
Nature
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| dates: |
1982
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| study type: | b cell assays |
| subject species: | Hepatitis B virus |
| fullName: |
G R Dreesman
Y Sanchez
I Ionescu-Matiu
J T Sparrow
H R Six
D L Peterson
F B Hollinger
J L Melnick
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| method: |
radio immuno assay (RIA)
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| name: |
Antibody to hepatitis B surface antigen after a single inoculation of uncoupled synthetic HBsAg peptides.
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| description: |
The peptide was made cyclic by formation of a S-S bridge between the 2 Cys residues. Also, a Lys residue was added to the N-terminus for linking purposes.
MIce were immunized with this peptide (or with a longer version, with 6 extra residues at the N-ter) in different adjuvants, with similar results in the recognition of HBsAg by the AUSAB radioimmunoassay in all groups, including the controls immunized with denatured HBsAg. These fingding suggest that a single dose without carrier of a structurally constrained peptide is able to elicit a significant response, comparable to that of the complete protein, with no major impact of the adjuvant used.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
