Mapping of immunodominant B-cell epitopes and the human serum albumin-binding site in natural hepatitis B virus surface antigen of defined genosubtype.

Title:

Mapping of immunodominant B-cell epitopes and the human serum albumin-binding site in natural hepatitis B virus surface antigen of defined genosubtype.

Dataset

identifier:	1369264
description:	epitope description:VRGLYFP antigen name:Large envelope protein host organism:Mus musculus BALB/c antibody name:2-12F2
aggregation:	instance of dataset
availability:	available
primaryPublications:	10644835

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1003444
landingPage:	http://www.iedb.org/assay/1369264

Publication

type:	Literature
publicationVenue:	J Gen Virol
dates:	2000

Dimension

study type:	b cell assays
subject species:	Hepatitis B virus ayw2

Person

fullName:	D Sobotta I Sominskaya J Jansons H Meisel S Schmitt K H Heermann G Kaluza P Pumpens W H Gerlich

Data Type

method:	western blot

Study

name:	Mapping of immunodominant B-cell epitopes and the human serum albumin-binding site in natural hepatitis B virus surface antigen of defined genosubtype.
description:	This peptide is conserved among the D/ayw2 and D/ayw3 genosubtypes. A/adw2 genosubtype differs in a single substitution, F6L, in the corresponding sequence. By a combination of peptide ELISA, fragment WB, and trypsin cleavage of Middle HBsAg, the epitope recognized by mAb 2-12F2 was mapped to these residues. Similar reactivity towards the A/adw2 HBsAg was seen, suggesting the F6L variation does not affect the epitope recognition.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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