Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1003467 |
| landingPage: |
http://www.iedb.org/assay/1355709 |
| type: |
Literature
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| publicationVenue: |
J Immunol Methods
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| dates: |
2001
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| study type: | b cell assays |
| subject species: |
| fullName: |
F D'Mello
C R Howard
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| method: |
western blot
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| name: |
An improved selection procedure for the screening of phage display peptide libraries.
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| description: |
This peptide was selected from a 15-mer random library by its ability to bind mAb 18/4, which recognizes residues 28-37 of the pre-S1 region of HBsAg.
This peptide, as others with homology to the DPAF residues of HBsAg (positions 31-34), was selected by panning of a library with mAb 18/7 directly to polystyrene beads, which proved to be the most effective method (compared to direct linking to latex beads and streptavidin-biotin linking to polystyrene plates) to obtain consensus-bearing mimotopes. Its reactivity was confirmed by immunoblot.
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| name: |
iedb
|
| homePage: |
http://www.iedb.org |
