HBV infection of cell culture: evidence for multivalent and cooperative attachment. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

HBV infection of cell culture: evidence for multivalent and cooperative attachment.

Dataset

identifier:	1357015
description:	epitope description:DPAF antigen name:Large envelope protein host organism:Mus musculus antibody name:mAb 18/7
aggregation:	instance of dataset
availability:	available
primaryPublications:	11500372

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1003459
landingPage:	http://www.iedb.org/assay/1357015

Publication

type:	Literature
publicationVenue:	EMBO J
dates:	2001

Dimension

study type:	b cell assays
subject species:	Hepatitis B virus

Person

fullName:	N Paran B Geiger Y Shaul

Data Type

method:	western blot

Study

name:	HBV infection of cell culture: evidence for multivalent and cooperative attachment.
description:	The mAb 18/7 generated against the larger preS1 21-47 (PLGFFPDHQLDPAFGANSNNPDWDFNPGK) bound preferentially to the epitope present in the wild-type HBV preS1. MAb 18/7 failed to bind mutant preS1 having the altered sequence ALQPDF. Moreover, the mutant having the scrambled QLDPAF to ALQPDF motif showed poor attachment to hepatocytes cells following conjugation to beads in BMA assays. This suggests that the epitope is the site that dominates HBV attachment and mAb binding. This motif is also present in the HBV X protein.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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