Fine and domain-level epitope mapping of botulinum neurotoxin type A neutralizing antibodies by yeast surface display.

Title:

Fine and domain-level epitope mapping of botulinum neurotoxin type A neutralizing antibodies by yeast surface display.

identifier:	1368116
description:	epitope description:Q1254, F1255, N1256 antigen name:Botulinum neurotoxin type A (UniProt:A5HZZ9) host organism:Mus musculus BALB/c antibody name:S25
aggregation:	instance of dataset
availability:	available
primaryPublications:	17059824

study type:	b cell assays
subject species:	Clostridium botulinum A str. Hall

fullName:	R Levy C M Forsyth S L LaPorte I N Geren L A Smith J D Marks

method:	flow cytometry

name:	Fine and domain-level epitope mapping of botulinum neurotoxin type A neutralizing antibodies by yeast surface display.
description:	These residues are located in the C-terminal subdomain of the binding (Hc) domain of BoNT/A, adjacent to the ganglioside binding site. This is the BoNT/A1 toxin. A yeast-display system was used to identify residues involved in binding to the conformation-dependent mAb S25 by loss of binding to mutants, where BoNT/A1 Hc residues were replaced by the respective BoNT/A2 residues based on the fact that this mAb fails to recognize BoNT/A2.

name:	iedb
homePage:	http://www.iedb.org

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