Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1369686 |
| description: |
epitope description:2-O-methyl-4-(3-hydroxy-3-methylbutamido)-4,6-dideoxy-beta-D-glucosyl-(1->3)-alpha-L-rhamnosyl-(1->2)-alpha-L-rhamnosyl group
antigen name:Collagen triple helix repeat domain protein
host organism:Oryctolagus cuniculus New Zealand White
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| aggregation: |
instance of dataset
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| availability: |
available
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| primaryPublications: |
17133642 |
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1003556 |
| landingPage: |
http://www.iedb.org/assay/1369686 |
| type: |
Literature
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| publicationVenue: |
Chemistry
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| dates: |
2006
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| study type: | b cell assays |
| subject species: | Bacillus anthracis str. Sterne 34F2 |
| fullName: |
Alok S Mehta
Elke Saile
Wei Zhong
Therese Buskas
Russell Carlson
Elmar Kannenberg
Yvonne Reed
Conrad P Quinn
Geert-Jan Boons
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| method: |
ELISA
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| name: |
Synthesis and antigenic analysis of the BclA glycoprotein oligosaccharide from the Bacillus anthracis exosporium.
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| description: |
The structure depicted corresponds to 2-O-methyl-4-(3-hydroxy-3-methylbutamido)-4,6-dideoxy-beta-d-glucopyranosyl-(1&rarr
3)-alpha-L-rhamnopyranosyl-(1&rarr
3)-alpha-L-rhamnopyranosyl, which is part of the O-linked oligosaccharide subsitutuent of the glycoportein BclA found in B. anthracis exosporium. The non-reducing sugar, 2-O-methyl-4-(3-hydroxy-3-methylbutamido)-4,6-dideoxy-beta-d-glucopyranose, is a previously unknown saccharide found in B. anthracis and therefore called anthrose. The BclA (collagen-like) glycoprotein is found in B. anthracis exosporium, and it has a repeating region of X-X-Gly motif, which usually has a Thr residue that is a potential glycosylation site. The glycoprotein oligosaccharide moiety is studied here.
The oligosaccharide is recognized as a KLH-conjugate in direct ELISA, and in its free form in competitive inhibition ELISA, by the serum of rabbits immunized with either live or irradiated B.anthracis spores (but more strongly in the former case). By using analogs with different moieties at C4 and C2 of the anthrose terminal sugar, the C2 methyl substituent and the OH- group at the butyryl substitutent of the C4 are found dispensable for recognition, but the 3-methylbutytyl substituent itself is an important structural motif for the specific recognition of the trisaccharide.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
