Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1003928 |
| landingPage: |
http://www.iedb.org/assay/1374402 |
| type: |
Literature
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| publicationVenue: |
Infect Immun
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| dates: |
2001
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| study type: | b cell assays |
| subject species: |
| fullName: |
T A Pitcovsky
J Mucci
P Alvarez
M S Leguizamó
n
O Burrone
P M Alzari
O Campetella
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| method: |
western blot
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| name: |
Epitope mapping of trans-sialidase from Trypanosoma cruzi reveals the presence of several cross-reactive determinants.
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| description: |
The epitope is homologous to T. cruzi trans-sialidase residues residues 70-77, 494-501 and 526-534. Residues 70-77 contains a characteristic Asp box (SXDXGXTW) of microbial sialidases. Residues 523-538 form a highly flexible loop of the lectin-like domain.
Sera from mice immunized with phage particles displaying the epitope reacted with peptide 1441 (WQPIYGSTPVTPTGSC) based on residues 524-538 of T. cruzi trans-sialidase with an added C-terminal Cys. This is one region to which the epitope/mimotope has sequence homology.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
