Successful use of oligopeptides as immunogens in the preparation of antisera to immediate-early gene products of herpes simplex virus type 1.

Title:

Successful use of oligopeptides as immunogens in the preparation of antisera to immediate-early gene products of herpes simplex virus type 1.

identifier:	1377426
description:	epitope description:PAAGTDAGEDAG antigen name:Major viral transcription factor ICP4 host organism:Oryctolagus cuniculus antibody name:anti-E175 oligopeptide
aggregation:	instance of dataset
availability:	available
primaryPublications:	6202828

study type:	b cell assays
subject species:	Herpes simplex virus (type 1 / strain 17)

fullName:	J W Palfreyman J B Maclean E Messeder R C Sheppard

method:	immunoprecipitation

name:	Successful use of oligopeptides as immunogens in the preparation of antisera to immediate-early gene products of herpes simplex virus type 1.
description:	Antisera from rabbits immunized with the epitope conjugated to BSA immunoprecipitated the 175 kDa immediate-early gene product (IE175) from HSV-1-infected cells. The antisera also immunoprecipitated the early E136'143 protein, which the authors attributed to co-precipitation rather than a specific reaction. The antisera did not react with the HSV-2 IE175 equivalent protein of 182 kDa. Preincubation of the antisera with the epitope blocked recognition of IE175.

name:	iedb
homePage:	http://www.iedb.org

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