Heat treatment of serum and plasma induces false positive results in the antiphospholipid antibody ELISA.

Title:

Heat treatment of serum and plasma induces false positive results in the antiphospholipid antibody ELISA.

identifier:	1790275
description:	epitope description:cardiolipin host organism:Homo sapiens
aggregation:	instance of dataset
availability:	available
primaryPublications:	2099746

study type:	b cell assays
subject species:

fullName:	P Hasselaar D A Triplett A LaRue R H Derksen L Blokzijl P G de Groot D R Wagenknecht J A McIntyre

method:	ELISA

name:	Heat treatment of serum and plasma induces false positive results in the antiphospholipid antibody ELISA.
description:	All of the sera were negative when the ELISA was performed with unheated sera. Heating of these sera for 30 minutes at 56ºC resulted in a positive IgM anti-cardiolipin level in 5 sera tested. The phenomenon is dependent on duration and degree of heating. Pooled normal serum was also tested.

name:	iedb
homePage:	http://www.iedb.org

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