Magnitude of the hydrophobic effect at central versus peripheral sites in protein-protein interfaces.

Title:

Magnitude of the hydrophobic effect at central versus peripheral sites in protein-protein interfaces.

identifier:	1792328
description:	epitope description:H33, G34, D36, N37, Y38, R39, W81, R91, L93, T107, N111, K114, K115, S118, D119, G120 antigen name:Gal d 4 host organism:Mus musculus BALB/c antibody name:HyHEL-63 mutant Y33V
aggregation:	instance of dataset
availability:	available
primaryPublications:	15698573

study type:	b cell assays
subject species:	Gallus gallus

fullName:	Yili Li Yuping Huang Chittoor P Swaminathan Sandra J Smith-Gill Roy A Mariuzza

method:	calorimetry

name:	Magnitude of the hydrophobic effect at central versus peripheral sites in protein-protein interfaces.
description:	The epitope residues were calculated from [PDB: 1XGT, 1XGR] at 4Å distance. Epitopes in other mutant structures [PDB: 1XGP, 1XGQ, 1XGU] contain a few additional residues (see each curated structure for detail). Epitope specific HyHEL-63 Fab mutant Y33V bound to hen egg white lysozyme with about 100-fold reduced affinity compared to mutant Y33F (KA = 1.9 x 10⁸ 1/ M) and to wild type HyHEL-63 Fab (KA = 2.5 x 10⁸ 1/ M).

name:	iedb
homePage:	http://www.iedb.org

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.