Amino acids critical for binding of autoantibody to an immunodominant conformational epitope of the pyruvate dehydrogenase complex subunit E2: identification by phage display and site-directed mutagenesis.

Title:

Dataset

identifier:	1767473
description:	epitope description:H185, M186, F231, E232, V233 antigen name:Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial host organism:Homo sapiens
aggregation:	instance of dataset
availability:	available
primaryPublications:	16360019

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1017232
landingPage:	http://www.iedb.org/assay/1767473

Publication

type:	Literature
publicationVenue:	Mol Immunol
dates:	2006

Dimension

study type:	b cell assays
subject species:	Homo sapiens

Person

fullName:	Marita Scealy Ian R Mackay Merrill J Rowley

Data Type

method:	ELISA

Study

name:	Amino acids critical for binding of autoantibody to an immunodominant conformational epitope of the pyruvate dehydrogenase complex subunit E2: identification by phage display and site-directed mutagenesis.
description:	PBC sera reacted with the PDC-E2 protein containing the wild-type epitope. Mutant PDC-E2 proteins containing single or multiple alanine substitutions at the epitopic residues H132, M133, F178, E179, V180 abolished recognition of the protein, suggesting these amino acids as critical for autoantibody recognition.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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