Evidence that shed thyrotropin receptor A subunits drive affinity maturation of autoantibodies causing Graves' disease.

Title:

Evidence that shed thyrotropin receptor A subunits drive affinity maturation of autoantibodies causing Graves' disease.

identifier:	1768979
description:	epitope description:SSPPCE antigen name:Thyrotropin receptor host organism:Mus musculus BALB/c antibody name:KSAb1, KSAb2
aggregation:	instance of dataset
availability:	available
primaryPublications:	19066298

study type:	b cell assays
subject species:	Homo sapiens

fullName:	Yumiko Mizutori Chun-Rong Chen Francesco Latrofa Sandra M McLachlan Basil Rapoport

method:	flow cytometry

name:	Evidence that shed thyrotropin receptor A subunits drive affinity maturation of autoantibodies causing Graves' disease.
description:	The epitope was deduced. The authors deduced that the N-terminus of the TSHR ectodomain was important for mAb binding because the mAb did not bind to a chimeric TSH-LH receptor construct with residues 25-30 substituted with rat LHR residues 32-37 (PEPCD). Substitution of TSHR residues 261-418 with the LHR sequence did not significantly reduce binding. At lower Ab concentrations, the mAb preferentially bound to the GPI-anchored TSHR ectodomain expressed in CHO cells compared to the expressed wild type TSHR.

name:	iedb
homePage:	http://www.iedb.org

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