Definition of Brucella A and M epitopes by monoclonal typing reagents and synthetic oligosaccharides.

Title:

Definition of Brucella A and M epitopes by monoclonal typing reagents and synthetic oligosaccharides.

identifier:	1771377
description:	epitope description:Brucella sp. O-PS M-antigen antigen name:lipopolysaccharide host organism:Mus musculus BALB/c antibody name:Bm3-2, Bm3-7, Bm3-8
aggregation:	instance of dataset
availability:	available
primaryPublications:	2474505

study type:	b cell assays
subject species:	Brucella melitensis bv. 1 str. 16M

fullName:	D R Bundle J W Cherwonogrodzky M A Gidney P J Meikle M B Perry

method:	antigen inhibition

name:	Definition of Brucella A and M epitopes by monoclonal typing reagents and synthetic oligosaccharides.
description:	The M antigen is a homopolymer of 4,6-dideoxy-4-formamido-alpha-D-mannopyranose residues, where the repeating unit contains one alpha1,3-linked and four alpha1,2-linked monosaccharide residues. The concentration of O polysaccharide needed to achieve 50% inhibition of binding of the epitope-specific mAbs to the B. melitensis O-PS was determined in competitive ELISA using monoclonal antibodies previously shown to bind to the epitope. The antibodies bind weakly to Y. enterocolitica O polysaccharide.

name:	iedb
homePage:	http://www.iedb.org

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