Identification of a thrombin-interactive site within the FVIII A2 domain that is responsible for the cleavage at Arg372.

Title:

Identification of a thrombin-interactive site within the FVIII A2 domain that is responsible for the cleavage at Arg372.

identifier:	1773004
description:	epitope description:RPLYSRRLPKGVKHLKDFPILPGEIF antigen name:Coagulation factor VIII host organism:Mus musculus antibody name:mAb413
aggregation:	instance of dataset
availability:	available
primaryPublications:	18081893

study type:	b cell assays
subject species:	Homo sapiens

fullName:	Keiji Nogami Evgueni L Saenko Masahiro Takeyama John C Giddings Akira Yoshioka Midori Shima

method:	biological activity

name:	Identification of a thrombin-interactive site within the FVIII A2 domain that is responsible for the cleavage at Arg372.
description:	The sequence of the epitope was provided in cited reference Healey et al. J Biol Chem 1995 270:14,505-14,509 [PMID: 7540171]. The epitope-specific anti-A2 monoclonal antibody mAb413 demonstrated a dose-dependent inhibition of thrombin-catalyzed activation of FVIII. In a separate assay, time-course of heavy chain cleavage analyzed by blotting showed that with mAb413, cleavage at R372 was markedly diminished. This was consistent with the effect of mAb413 on thrombin-catalyzed activation of the pro-co-factor and indicate that the region comprising the anti-A2 mAb epitope is likely to contain with the thrombin-interactive site(s) that affects the cleavage at R372. in the heavy chain. The Ab also inhibited the binding of A2 to Ah-thrombin.

name:	iedb
homePage:	http://www.iedb.org

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.