Binding of antiphospholipid antibodies to discontinuous epitopes on domain I of human beta(2)-glycoprotein I: mutation studies including residues R39 to R43.

Title:

Binding of antiphospholipid antibodies to discontinuous epitopes on domain I of human beta(2)-glycoprotein I: mutation studies including residues R39 to R43.

Dataset

identifier:	1779804
description:	epitope description:RGGMR antigen name:Beta-2-glycoprotein 1 host organism:Homo sapiens Caucasian antibody name:APS patient sera
aggregation:	instance of dataset
availability:	available
primaryPublications:	17195232

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1016188
landingPage:	http://www.iedb.org/assay/1779804

Publication

type:	Literature
publicationVenue:	Arthritis Rheum
dates:	2007

Dimension

study type:	b cell assays
subject species:	Homo sapiens

Person

fullName:	Yiannis Ioannou Charis Pericleous Ian Giles David S Latchman David A Isenberg Anisur Rahman

Data Type

method:	antigen inhibition

Study

name:	Binding of antiphospholipid antibodies to discontinuous epitopes on domain I of human beta(2)-glycoprotein I: mutation studies including residues R39 to R43.
description:	The epitope sequence was preovide in cite reference Ioannou et al. BMC Biotechnol 2006 6:8 [PMID: 16472380]. A panel of purified IgG samples derived from APS patients was selected. Direct binding (10/10) and inhibition of binding (8/8) was observed. The epitope was deduced using mutants of the wild-type domain I sequence. The greatest reduction in binding compared with wild-type domain I was observed with the single-point mutant R39S.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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