Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1783629 |
| description: |
epitope description:R32, H33, G34, N37, Y38, R39, W80, W81, R91, L93, N95, T107, N111, K114, K115, I116, S118, D119, G120
antigen name:Gal d 4
host organism:Mus musculus BALB/c
antibody name:hHyHEL-10 Fv
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
18227432 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1011922 |
| landingPage: |
http://www.iedb.org/assay/1783629 |
| type: |
Literature
|
| publicationVenue: |
Protein Sci
|
| dates: |
2008
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| study type: | b cell assays |
| subject species: | Gallus gallus |
| fullName: |
Takeshi Nakanishi
Kouhei Tsumoto
Akiko Yokota
Hidemasa Kondo
Izumi Kumagai
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| method: |
calorimetry
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| name: |
Critical contribution of VH-VL interaction to reshaping of an antibody: the case of humanization of anti-lysozyme antibody, HyHEL-10.
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| description: |
The epitope residues were calculated based on the 4 Å
atomic distance between the antigen and the antibody from [PDB: 2EKS].
Humanization of the epitope-specific murine antibody HyHEL-10 led to 10-fold reduced affinity for hen egg white lysozyme (HEL) at 30ºC. The affinity of parent murine HyHyHEL-10 was 8.21 x 108 M-1. Affinity was determined by isothermal titration calorimetry at four temperatures (20ºC, 25ºC, 30ºC, and 35ºC).
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| name: |
iedb
|
| homePage: |
http://www.iedb.org |
