Binding of monoclonal antibodies to the movement protein (MP) of Tobacco mosaic virus: influence of subcellular MP localization and phosphorylation.

Title:

Binding of monoclonal antibodies to the movement protein (MP) of Tobacco mosaic virus: influence of subcellular MP localization and phosphorylation.

Dataset

identifier:	1784895
description:	epitope description:SEATVAESDSF antigen name:Movement protein host organism:Mus musculus antibody name:7D9, 8D12
aggregation:	instance of dataset
availability:	available
primaryPublications:	20164264

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1019799
landingPage:	http://www.iedb.org/assay/1784895

Publication

type:	Literature
publicationVenue:	J Gen Virol
dates:	2010

Dimension

study type:	b cell assays
subject species:	Tobacco mosaic virus

Person

fullName:	Lidia G Tyulkina Elena M Karger Anna A Sheveleva Joseph G Atabekov

Data Type

method:	western blot

Study

name:	Binding of monoclonal antibodies to the movement protein (MP) of Tobacco mosaic virus: influence of subcellular MP localization and phosphorylation.
description:	The epitope-specific mAb bound to recombinant MP, but did not bind to TMV movement protein in the cell wall and ER fractions isolated from N. benthamiana cells infected with TMV, not did it recognize MP extracted from N. benthamiana or N. tabacum cells. Mutational analysis revealed that residues S1, T4, S8, and S10 were critical for binding. Phosphorylation of MP decreased the response.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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