Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1787454 |
| description: |
epitope description:K31, R32, H33, G34, D36, N37, Y38, R39, W81, R91, L93, N95, T107, N111, K114, K115, I116, S118, D119, G120, N121
antigen name:Gal d 4
host organism:Mus musculus BALB/c
antibody name:HyHEL-63
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| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
12515535 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1019323 |
| landingPage: |
http://www.iedb.org/assay/1787454 |
| type: |
Literature
|
| publicationVenue: |
Biochemistry
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| dates: |
2003
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| study type: | b cell assays |
| subject species: | Gallus gallus |
| fullName: |
Yili Li
Mariela Urrutia
Sandra J Smith-Gill
Roy A Mariuzza
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| method: |
x-ray crystallography
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| name: |
Dissection of binding interactions in the complex between the anti-lysozyme antibody HyHEL-63 and its antigen.
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| description: |
The epitope residues were calculated based on the 4 Å
atomic distance between the antigen and the antibody from [PDB: 1NBY]. The lysine residues at positions 696 (96) and 697 (97) (residues 114 and 115 in GenBank # 126608) were individually substituted with alanine.
The epitope of HyHEL-63 bound to hen egg white lysozyme, with alanine substituted residue K96A (A696) (HEL K96A; GI: 29726605), was solved from the crystal structure by molecular replacement. The affinity of HyHEL-63 for HEL K96A was reduced approximately 33,000-fold (KA = 1.1 x 104 1/M) compared to wild type HEL (KA = 3.6 x 108 1/M).
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| name: |
iedb
|
| homePage: |
http://www.iedb.org |
