A mutation designed to alter crystal packing permits structural analysis of a tight-binding fluorescein-scFv complex.

Title:

A mutation designed to alter crystal packing permits structural analysis of a tight-binding fluorescein-scFv complex.

identifier:	1786625
description:	epitope description:fluorescein host organism:Homo sapiens antibody name:FITC-E2 scFV
aggregation:	instance of dataset
availability:	available
primaryPublications:	16195545

study type:	b cell assays
subject species:

fullName:	Annemarie Honegger Silvia Spinelli Christian Cambillau Andreas Plü ckthun

method:	binding assay

name:	A mutation designed to alter crystal packing permits structural analysis of a tight-binding fluorescein-scFv complex.
description:	The binding affinity of scFv FITC-E2 for fluorescein was determined by fluorescence titration. Alanine scanning of the heavy and light chain CDR regions determined the residue substitutions that resulted in a loss of binding, only some of which corresponded to contact residues in the scFV-hapten crystal structure.

name:	iedb
homePage:	http://www.iedb.org

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.