Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition.

Title:

Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition.

identifier:	1786885
description:	epitope description:alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo antigen name:lipopolysaccharide host organism:Mus musculus BALB/c antibody name:S73-2
aggregation:	instance of dataset
availability:	available
primaryPublications:	20000757

study type:	b cell assays
subject species:	Chlamydiae

fullName:	Cory L Brooks Sven Mü ller-Loennies Svetlana N Borisova Lore Brade Paul Kosma Tomoko Hirama C Roger Mackenzie Helmut Brade Stephen V Evans

method:	surface plasmon resonance (SPR)

name:	Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition.
description:	The solution affinity of S73-2 Fab for the epitope was determined by surface plasmon resonance using inhibition of Fab binding to immobilized Kdo(2->8)Kdo(2->4)Kdo-BSA. A similar value (KD = 300 nM) was determined for S73-2 Fab bound to immobilized epitope-BSA conjugate by SPR. Fab S73-2 also bound to the epitope-BSA conjugate by ELISA.

name:	iedb
homePage:	http://www.iedb.org

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