Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1019560 |
| landingPage: |
http://www.iedb.org/assay/1787963 |
| type: |
Literature
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| publicationVenue: |
Int Arch Allergy Appl Immunol
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| dates: |
1990
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| study type: | b cell assays |
| subject species: | Dermatophagoides pteronyssinus |
| fullName: |
W K Greene
K Y Chua
G A Stewart
W R Thomas
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| method: |
radio immuno assay (RIA)
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| name: |
Antigenic analysis of group I house dust mite allergens using random fragments of Der p I expressed by recombinant DNA libraries.
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| description: |
The epitope was deduced.
Rabbit antiserum against the related species, D. farinae, was absorbed with each of seven fusion proteins containing overlapping peptides. The changes in reactivity of antigenic peptides after absorption was assessed using dot blot radioimmunoassay and the results were used to deduce regions responsible for antibody reactivity. The epitope, region 60-72, was inferred because absorption of 60-111 abrogated reactivity to the cross-absorptive peptides 48-72 and 57-81.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
