Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1007712 |
| landingPage: |
http://www.iedb.org/assay/1474025 |
| type: |
Literature
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| publicationVenue: |
Clin Microbiol Infect
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| dates: |
2008
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| study type: | b cell assays |
| subject species: | Enterovirus 5865/sin/000009 |
| fullName: |
D G W Foo
R X Ang
S Alonso
V T K Chow
S H Quak
C L Poh
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| method: |
western blot
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| name: |
Identification of immunodominant VP1 linear epitope of enterovirus 71 (EV71) using synthetic peptides for detecting human anti-EV71 IgG antibodies in Western blots.
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| description: |
Epitope mapping was performed using overlapping peptides and serum samples collected from children attending a pediatric outpatient clinic at the National University Hospital of Singapore. The samples were screened for the presence of anti-Enterovirus 71-neutralizing antibodies. In a further experiment, the most reactive peptide containing the core epitope region (DLPLEGTTNPNGYAN) was tested as a capture antigen for human anti-Enterovirus 71in ELISA and western blot assays. No significant immunoreactivity was detected by ELISA, while reactivity was detected in western blots.
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| name: |
iedb
|
| homePage: |
http://www.iedb.org |
