Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/315370 |
| landingPage: |
http://www.iedb.org/assay/1475450 |
| type: |
Literature
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| publicationVenue: |
Int Arch Allergy Immunol
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| dates: |
2000
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| study type: | b cell assays |
| subject species: | Glycine max |
| fullName: |
R M Helm
G Cockrell
C Connaughton
H A Sampson
G A Bannon
V Beilinson
N C Nielsen
A W Burks
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| method: |
western blot
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| name: |
A soybean G2 glycinin allergen. 2. Epitope mapping and three-dimensional modeling.
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| description: |
Data originally imported from the Database of Functional Molecular Immunology, FIMM (http://sdmc.lit.org.sg:8080/fimm/) and recurated. The following refers to the epitope id as found in the original FIMM database: P001866
The epitope sequence was determined through study of overlapping 15mer peptides spanning the antigenic region, residues 57-111. The epitope was considered immunodominant since it was recognized by sera from 6 of 7 soybean-sensitive individuals. Single amino acid alanine substitutions at positions 7, 10, and 13-15 abloished IgE binding to the peptide. Structural modeling of G2 glycinin based on the known 3-D structure of beta-phaseolin predict the epitope to be located on the surface of the trimeric G2 molecule.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
