A soybean G2 glycinin allergen. 2. Epitope mapping and three-dimensional modeling. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

A soybean G2 glycinin allergen. 2. Epitope mapping and three-dimensional modeling.

Dataset

identifier:	1475467
description:	epitope description:QHTFNLKSQQARQVK antigen name:Glycinin G2 host organism:Homo sapiens antibody name:human sera
aggregation:	instance of dataset
availability:	available
primaryPublications:	11112857

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/315370
landingPage:	http://www.iedb.org/assay/1475467

Publication

type:	Literature
publicationVenue:	Int Arch Allergy Immunol
dates:	2000

Dimension

study type:	b cell assays
subject species:	Glycine max

Person

fullName:	R M Helm G Cockrell C Connaughton H A Sampson G A Bannon V Beilinson N C Nielsen A W Burks

Data Type

method:	western blot

Study

name:	A soybean G2 glycinin allergen. 2. Epitope mapping and three-dimensional modeling.
description:	Data originally imported from the Database of Functional Molecular Immunology, FIMM (http://sdmc.lit.org.sg:8080/fimm/) and recurated. The following refers to the epitope id as found in the original FIMM database: P001875 The epitope sequence was determined through study of overlapping 15mer peptides spanning the antigenic region, residues 449-471. The epitope was considered immunodominant since it was recognized by sera from 5 of 7 soybean-sensitive individuals. Single amino acid alanine substitutions did not affect IgE binding. Structural modeling of G2 glycinin based on the known 3-D structure of beta-phaseolin predict the epitope to be located on the surface of the trimeric G2 molecule.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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