IgE and IgG cross-reactivity among Lol p I and Lol p II/III. Identification of the C-termini of Lol p I, II, and III as cross-reactive structures.

Title:

IgE and IgG cross-reactivity among Lol p I and Lol p II/III. Identification of the C-termini of Lol p I, II, and III as cross-reactive structures.

Dataset

identifier:	1476300
description:	epitope description:KGGMKNVFDEVIPTAFTVGKTYTPEYN antigen name:Lol p 3 host organism:Oryctolagus cuniculus antibody name:rabbit anti-Lol p I serum
aggregation:	instance of dataset
availability:	available
primaryPublications:	7518655

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1005603
landingPage:	http://www.iedb.org/assay/1476300

Publication

type:	Literature
publicationVenue:	Allergy
dates:	1994

Dimension

study type:	b cell assays
subject species:	Lolium perenne

Person

fullName:	R van Ree W A van Leeuwen M van den Berg H H Weller R C Aalberse

Data Type

method:	antigen inhibition

Study

name:	IgE and IgG cross-reactivity among Lol p I and Lol p II/III. Identification of the C-termini of Lol p I, II, and III as cross-reactive structures.
description:	At high concentrations, the epitope or purified Lol p III inhibited rabbit anti-Lol p I serum from binding to the homologous C-terminal peptide residues 216-240 (EGGTKSEVEDVIPEGWKADTSYSAK) from Lol p I coupled to Sepharose. Using overlapping peptides from the C-terminus of Lol p III, the region responsible for cross-reactivity was mapped to residues 66-80 (FRFLSKGGMKNVFDEF). Lol p III (81-97) also inhibited binding to Lol p I (216-240). The authors attribute the inhibition by Lol p III to contamination with Lol p I because of the high concentration required.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.