Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1006581 |
| landingPage: |
http://www.iedb.org/assay/1476298 |
| type: |
Literature
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| publicationVenue: |
Vaccine
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| dates: |
2002
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| study type: | b cell assays |
| subject species: | Foot-and-mouth disease virus (strain A12) |
| fullName: |
Chang Yi Wang
Tseng Yuan Chang
Alan M Walfield
John Ye
Ming Shen
Shih Ping Chen
Ming Chang Li
Yeou Liang Lin
Ming Hwa Jong
Ping Cheng Yang
Nancy Chyr
Ed Kramer
Fred Brown
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| method: |
biological activity
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| name: |
Effective synthetic peptide vaccine for foot-and-mouth disease in swine.
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| description: |
The epitope sequence was confirmed by the author. The amino acid starting and ending positions for this epitope refer to the positions for the homologous loop structure in the VP1 protein of the O-type virus, which is longer by one amino acid than the corresponding A sequence. The amino acid positions of the polyprotein, from which the VP1 is processed, are referenced in the database sequence.
Animals immunized with this epitope plus carrier had a TCID50 of 105.5, three logs higher than immunization without carrier, indicating that the carrier contributed to the antigenicity of this epitope. Immunization with similar constructs containing a longer version of the epitope gave better neutralization activity. Animals immunized with the mutated cyclized version of the epitope, A12{134(N-C)-158(Q-C)-159}, plus carrier had a TCID50 of 107, indicating that mutation and cyclization improved the response to this epitope.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
