Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | Monoclonal antibody to shiga toxin 1, which blocks receptor binding and neutralizes cytotoxicity. |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1008065 |
| landingPage: |
http://www.iedb.org/assay/1478062 |
| type: |
Literature
|
| publicationVenue: |
Microbiol Immunol
|
| dates: |
2002
|
| study type: | b cell assays |
| subject species: | Enterobacteria phage 933J |
| fullName: |
Hiroshi Nakao
Chiwa Kataoka
Nobutaka Kiyokawa
Junichiro Fujimoto
Shinji Yamasaki
Tae Takeda
|
| method: |
biological activity
|
| name: |
Monoclonal antibody to shiga toxin 1, which blocks receptor binding and neutralizes cytotoxicity.
|
| description: |
The sequence is from the reference cited: Jackson et al. (1987) Microb Pathog 2: 147-153. [PMID: 3333796]
MAb 5-5B neutralized the cytotoxicity of Stx1 and Stx mutants T1A and G25A, but not N55T, indicating that residue N55 was an epitopic determinant of the antibody. MAb 5-5B was shown to bind Stx1 B subunits and inhibit the binding of Stx1 to Ramos cells. MAb 5-5B did not bind to cell-bound Stx1.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |