| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1235 |
| landingPage: |
http://www.iedb.org/assay/1478910 |
| type: |
Literature
|
| publicationVenue: |
Biochem Biophys Res Commun
|
| dates: |
2003
|
| study type: | b cell assays |
| subject species: | Plum pox virus |
| fullName: |
Olga Esteban
Juan Antonio Garcí
a
Marí
a Teresa Gorris
Elvira Domí
nguez
Mariano Cambra
|
| method: |
western blot
|
| name: |
Generation and characterisation of functional recombinant antibody fragments against RNA replicase NIb from plum pox virus.
|
| description: |
The antibody construct scFv2A and the parent mAb 2A detected a 59 kDa protein, the expected size of NIb, on Western blots of PPV-infected plant samples, but not healthy plant samples. The construct scFv2A and mAb 2A also recognized PPV-NIb in the native state in virus-infected plants as analyzed by direct tissue print-ELISA.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |