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identifier: 1478910
description:
epitope description:YLEAFY
antigen name:Genome polyprotein
host organism:Mus musculus BALB/c
antibody name:scFv2A
aggregation:
instance of dataset
availability:
available
primaryPublications: 12535657
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1235
landingPage: http://www.iedb.org/assay/1478910
type:
Literature
publicationVenue:
Biochem Biophys Res Commun
dates:
2003
study type: b cell assays
subject species: Plum pox virus
fullName:
Olga Esteban
Juan Antonio Garcí
a
Marí
a Teresa Gorris
Elvira Domí
nguez
Mariano Cambra
method:
western blot
name:
Generation and characterisation of functional recombinant antibody fragments against RNA replicase NIb from plum pox virus.
description:
The antibody construct scFv2A and the parent mAb 2A detected a 59 kDa protein, the expected size of NIb, on Western blots of PPV-infected plant samples, but not healthy plant samples. The construct scFv2A and mAb 2A also recognized PPV-NIb in the native state in virus-infected plants as analyzed by direct tissue print-ELISA.

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