Generation and characterisation of functional recombinant antibody fragments against RNA replicase NIb from plum pox virus.

Title:

Generation and characterisation of functional recombinant antibody fragments against RNA replicase NIb from plum pox virus.

identifier:	1478911
description:	epitope description:YLEAFY antigen name:Genome polyprotein host organism:Mus musculus BALB/c antibody name:2A
aggregation:	instance of dataset
availability:	available
primaryPublications:	12535657

study type:	b cell assays
subject species:	Plum pox virus

fullName:	Olga Esteban Juan Antonio Garcí a Marí a Teresa Gorris Elvira Domí nguez Mariano Cambra

method:	western blot

name:	Generation and characterisation of functional recombinant antibody fragments against RNA replicase NIb from plum pox virus.
description:	The epitope of mAb 2A was determined to be within the C-terminal region (residues 476-518) of NIb by Western blot analysis of deletion mutants. The epitope was further deduced through study of overlapping peptides covering the region 479-517. The minimal epitope (YLEAFY) was assigned based on the region of overlap. Antibody construct scFv2A also reacted with peptides lacking the Tyr and Leu in positions 1 and 2 of the epitope, indicating the residues are not essential.

name:	iedb
homePage:	http://www.iedb.org

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