Epitope mapping porcine reproductive and respiratory syndrome virus by phage display: the nsp2 fragment of the replicase polyprotein contains a cluster of B-cell epitopes.

Title:

Epitope mapping porcine reproductive and respiratory syndrome virus by phage display: the nsp2 fragment of the replicase polyprotein contains a cluster of B-cell epitopes.

Dataset

identifier:	1479231
description:	epitope description:QPLNLSLAAWP antigen name:Replicase polyprotein 1ab host organism:Sus scrofa antibody name:42-dpi
aggregation:	instance of dataset
availability:	available
primaryPublications:	11238854

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1006592
landingPage:	http://www.iedb.org/assay/1479231

Publication

type:	Literature
publicationVenue:	J Virol
dates:	2001

Dimension

study type:	b cell assays
subject species:	Porcine respiratory and reproductive syndrome virus 111/92

Person

fullName:	M B Oleksiewicz A Bø tner P P Normann T Storgaard

Data Type

method:	ELISA

Study

name:	Epitope mapping porcine reproductive and respiratory syndrome virus by phage display: the nsp2 fragment of the replicase polyprotein contains a cluster of B-cell epitopes.
description:	The epitope sequence is identical in Lelystad virus, also known as porcine respiratory and reproductive syndrome virus strain Lelystad. The epitope sequence was selected from a phage display library using 3 or 4 rounds of selection with day 42 postinfection (42-dpi) swine serum. It was confirmed that the plaque-purified phage clone reacted with 42-dpi sera in an ELISA of total porcine Ig. Seroconversion toward the epitope occurred late, > 21 days postinfection, but seroconversion rates were not predictive of titers.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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